Add to Basket
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

CD8a Molecule (CD8A) antibody (PerCP)

Antigen

CD8a Molecule (CD8A)
Synonyms CD8, MAL, p32, Leu2, Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, MGC136643, zgc:136643, cd8, LOC100136450
Clonality Monoclonal (MEM-31)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives PerCP
Application
Alternatives Flow Cytometry (FACS)
3 references available
Catalog no. ABIN302050
Quantity 100 tests
Price 377.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 3 to 5 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name CD8
Immunogen Crude thymus membrane fraction.
Isotype IgG2a
Clone MEM-31
Description The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/betaheterodimer on majority of MHC I-restricted conventional T cells and thymocytes and asalpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cellsand dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an importantmechanism to control their effector function. Assembly of CD8 alpha-beta but notalpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruitingtriggered TCR complexes to these membrane microdomains as well as affinity of TCR toMHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cellsurface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cellinteractions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).The antibody does not react with formaldehyde-fixed cells, negative in WesternBlotting application.HLDA III, WS Code T 575

Application Details
Publications Alternatives back to top

Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Storage Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Publications Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Estefanuia, Flores, Guomez-Lozano et al.: "Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4402-10, 2007 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "CD8a Molecule (CD8A)", type "Antibodies"
Hosts Mouse (310), Rat (106), Rabbit (82)
Reactivities Human (288), Mouse (Murine) (121), Rat (Rattus) (50), Cat (Feline) (21), Chicken (19), Dog (Canine) (14), Pig (Porcine) (13), Cow (Bovine) (12), Monkey (10), Sheep (Ovine) (10), Horse (Equine) (8), Rabbit (8), Guinea Pig (5), Turkey (5), Goat (4), Duck (1)
Applications Flow Cytometry (FACS) (387), Immunohistochemistry (Frozen Sections) (IHC (fro)) (131), Immunoprecipitation (IP) (76), Immunofluorescence (IF) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Western Blotting (WB) (43), ELISA (33), Immunohistochemistry (IHC) (21), Immunocytochemistry (ICC) (15), Functional Studies (Func) (7), Blocking Antibody (Inhibition) (6), Cytotoxicity Test (CyTox) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Enzyme Immunoassay (EIA) (5), Immunoelectron Microscopy (IEM) (3), ELISA (Detection) (2), To be determined by user (TBD) (1)
Conjugates FITC (84), Biotin (47), PE (41), APC (17), RPE (12), Cy5 (9), PE,Cy7 (6), PE,Cy5 (5), SPRD (5), Alexa Fluor 488 (4), HRP (4), PE,Cy5.5 (4), APC,Cy7 (3), Alexa Flour 488 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5,PE (3), Cy5.5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), Pacific Blue (3), RBITC (3), Alexa Fluor 350 (2), Alexa Fluor 555 (2), PE,Texas Red (TR) (2), APC,Cy5.5 (1), Alexa Fluor 700 (1), Cy5.5,PE (1), Cy7,PE (1), PE,DY590 (1), PE,DY647 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RPE,Cy5 (1), RPE,Cy5.5 (1), RPE,Cy7 (1)
Epitopes C-Term (1), N-Term (1)