Tubulin gamma 1 (TUBG1) antibody

Antigen: Tubulin gamma 1 (TUBG1)

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Clonality: Monoclonal (TU-32)

Host: Mouse

Reactivity: Protozoa, Plant

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN302067

Additional Information

Alternative name: gamma-tubulin

Immunogen: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: TU-32

Description: The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor memberof tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures,however, serve to provide structural primer for initiation of microtubular nucleation andgrowth, thereby being crutial for microtubule-based cellular processes, above all for mitoticspindle formation. In animal cells, a center of microtubule organization is the centrosomecomposed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar materialcontaining gamma-tubulin. Formation of the mitotic spindle is preceded by duplication ofcentrosome during S phase. Before mitosis, both centrosomes increase their microtubulenucleation capacity and form two microtuble asters that are pushed apart from each other bythe forces of motor proteins associated at the microtubule surface.

Specificity: The antibody TU-32 recognizes an epitope (amino acids 434-449 in human) withinC-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton andmicrotubule organizing center (MTOC).

Synonyms: TUBG, GCP-1, TUBGCP1, Tubg, MGC8041, AI451582, AI503389, 1500010O08Rik, Tubg1, MGC55894, MGC174841, zgc:55894, Xgam, Tubg2, TUBG1, MGC142358, LOC542424, TubG1, TUBC, TUBG3, GAMMA-TUBULIN, tug1, Tb03.27F10.510, DKFZp468N0827

Application Details

Application Notes: Western Blotting - Recommended dilution: 0.5 µg/ml. Application note: excellent

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only