ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1) antibody

Antigen: ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1)

Synonyms: Centd2, MGC176140, mKIAA0782, 2410002L19Rik, CENTD2, KIAA0782, ARAP1, centd2, Ci-ArfGAP-8

Clonality: Monoclonal (ARAP1-2)

Host: Mouse

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN302078

Additional Information

Alternative name: ARAP1

Immunogen: His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: ARAP1-2

Description: ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAPand RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptorseems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling.ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) andis important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to theplasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types.ARAP1 also affects cell spreading.

Specificity: The antibody ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein.

Application Details

Application Notes: Western Blotting - Recommended dilution: 2 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling

Restrictions: For Research Use only

Images

Colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.