Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) antibody

Antigen: Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D)

Synonyms: SHIP, SHIP1, hp51CN, SIP-145, MGC104855, MGC142140, MGC142142, INPP5D, MGC157261, inpp5d, ship, ship1, MGC68993

Clonality: Monoclonal (SHIP-02)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN302082

Additional Information

Alternative name: SHIP-1

Immunogen: Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: SHIP-02

Description: SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5'inositol phosphatase thatregulates cell responses in lymphocytes and myeloid cells by hydrolyzing the secondmessenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitoryand activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokineand growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cellmigration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as ascaffold for the recruitment of other proteins to the plasma membrane.

Specificity: The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confinedto hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weightsof 110, 125, 130, 135 and 145 kDa.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 2-5 µg/ml. Positive control: human blood leukocytes. Application note: - Positive control: RAMOS human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. Application note: Reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western Blotting