Cytokeratin 10/13 antibody

Antigen: Cytokeratin 10/13

Clonality: Monoclonal (DE-K13)

Host: Mouse

Reactivity: Human, Cat (Feline)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN302093

Additional Information

Alternative name: Cytokeratin 10+13

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: DE-K13

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).

Specificity: The antibody DE-K13 reacts with Cytokeratin 10 (56.5 kDa) and Cytokeratin 13 (53 kDa).Cytokeratins are a member of intermediate filaments subfamily represented in epithelialtissues. DE-K13 recognizes only Cytokeratin 13 on formalin-fixed, paraffin-embedded tissuesections.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1-3 µg/ml. Immunohistochemistry (paraffin sections) - Staining technique: Standard ABC technique (DAB+). Recommended dilution: 10 µg/ml (1: 100) Positive tissue: tonsilPretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature, or Target RetrievalSolution - High pH. Application note: The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections) - Recommended dilution: Use at an assay dependent dilution.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemistry on paraffin-embedded sections (tonsil)