Intra-Acrosomal Protein antibody

Antigen: Intra-Acrosomal Protein

Clonality: Monoclonal (Hs-14)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN302102

Additional Information

Alternative name: Intra-Acrosomal Protein (Human, Mouse)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acidand subsequently used for immunization.

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: Hs-14

Description: One of the most frequent causes of man infertility is defective sperm acrosome. Thisdamage can be detected using antibodies against intra-acrosomal proteins. Besidesdiagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins canbe used for evaluation of the physiological state of sperm cells as well as for selection of asuitable method of fertilization in the laboratories of assisted reproduction.

Specificity: The antibody Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal proteinassociated with the membranes of the acrosomal vesicle.

Application Details

Application Notes: Immunocytochemistry - Recommended dilution: Purified Antibody: 10 µg/ml Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescenceanalysis of pathological sperms (excellent tool for laboratories of assisted reproduction whenoptimal method of fertilization is sought).

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and size-exclusion chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Restrictions: For Research Use only

Images

Immunofluorescence analysis of 220 kDa intra-acrosomal protein in acetone-permeabilized human sperms using monoclonal antibody Hs-14 (normal spermiogram).