RNase L Inhibitor (ABCE1) antibody

Antigen: RNase L Inhibitor (ABCE1)

Synonyms: RLI, OABP, ABC38, RNS4I, RNASEL1, RNASELI

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN302131

Additional Information

Alternative name: ABCE1/ RNAse L inhibitor

Gene ID: 6059, 24015 (mouse), 361390 (rat)

Immunogen: C-KLNSIKDVEQKK

Format: Affinity-purified

Description: All names and symbols: ABCE1, RNase L inhibitor, RLI, OABP, ABC38, RNS4I, RNASEL1, RNASELI, ATP-binding cassette, sub-family E (OABP), member 1, ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor, HP68. Official Symbol: ABCE1. RefSeq number(s): NP_002931.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 70kDa band observed in lysates of cell lines A431 (calculated MW of 67.6kDa according to NP_002931.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN302131 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Shichijo, Ishihara, Azuma et al.: "ABCE1, a member of ATP-binding cassette transporter gene, encodes peptides capable of inducing HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes in colon cancer patients." in: Oncology reports, Vol. 13, Issue 5, pp. 907-13, 2005 (PubMed).