Ankyrin 1, Erythrocytic (ANK1) antibody

Antigen: Ankyrin 1, Erythrocytic (ANK1)

Synonyms: ANK, SPH1, SPH2, ANK1, MGC155206, CHANK1, wu:fa09h06, zgc:101835, MGC107745, nb, Ank-1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN302139

Additional Information

Alternative name: Ankyrin 1 / ANK1

Gene ID: 286, 11733 (mouse), 306570 (rat)

Immunogen: C-QIVKRASLKRGKQ

Format: Affinity-purified

Description: All names and symbols: ANK1 , ankyrin 1, erythrocytic , ANK, SPH1, SPH2, ankyrin 1, ankyrin-1, erythrocytic, ankyrin-R. Official Symbol: ANK1 . RefSeq number(s): NP_000028.3, NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2

Specificity: This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2). Human, Mouse, Rat, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Dubreuil: "Functional links between membrane transport and the spectrin cytoskeleton." in: The Journal of membrane biology, Vol. 211, Issue 3, pp. 151-61, 2006 (PubMed).