ATP-Binding Cassette, Sub-Family A (ABC1), Member 8 (ABCA8) antibody

Antigen: ATP-Binding Cassette, Sub-Family A (ABC1), Member 8 (ABCA8)

Synonyms: KIAA0822, MGC163152, Abca8b, ABCA8, abca8a

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN302143

Additional Information

Alternative name: ABCA8

Gene ID: 10351

Immunogen: C-KEQELGDFEEDFD

Format: Affinity-purified

Description: All names and symbols: ABCA8, ATP-binding cassette, sub-family A (ABC1), member 8, KIAA0822, MGC163152, ATP-binding cassette, sub-family A member 8. Official Symbol: ABCA8. RefSeq number(s): NP_009099.1

Specificity: This antibody is expected to cross-react with the highly similar Human ABCA9, since the immunizing peptide differs from ABCA9 with only one residue (F8). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave an approx. 50-55kDa band in Human Brain (Cerebellum, Cerebral Cortex, Frontal Cortex), Heart, Skeletal Muscle and Testis lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 179kDa according to NP_009099.1. The 50-55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only