Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody

Antigen: Egl Nine Homolog 3 (C. Elegans) (EGLN3)

Synonyms: PHD3, HIFPH3, FLJ21620, MGC125998, MGC125999, Phd3, SM-20, AI505553, AI648162, 2610021G09Rik, zgc:77019, wu:fj78a08, EGLN3, PHD-3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN302149

Additional Information

Alternative name: EGLN3

Gene ID: 112399, 112407 (mouse), 54702 (rat)

Immunogen: C-RLGKYYVKERSK

Format: Affinity-purified

Description: All names and symbols: EGLN3 , egl nine homolog 3 (C. elegans) , FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3, EGL nine (C.elegans) homolog 3, egl nine homolog 3, egl nine-like protein 3 isoform. Official Symbol: EGLN3 . RefSeq number(s): NP_071356.1

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 30kDa band observed in Human Skeletal Muscle lysates (calculated MW of 27.7kDa according to NP_071356.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN302149 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Nakayama, Gazdoiu, Abraham et al.: "Hypoxia-induced assembly of prolyl hydroxylase PHD3 into complexes: implications for its activity and susceptibility for degradation by the E3 ligase Siah2." in: The Biochemical journal, Vol. 401, Issue 1, pp. 217-26, 2006 (PubMed).