Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3) antibody

Antigen: Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3)

Synonyms: EAA5, GLR7, GLUR7, GluR7a, Glur7, Glur-7, GluR7-3, 9630027E11, GRIK3, eaa5, glr7, glur7

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus), Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN302159

Additional Information

Alternative name: GRIK3 / GLUR7

Gene ID: 2899, 14807 (mouse), 298521 (rat)

Immunogen: C-KIRQLPIDSDDSRP

Format: Affinity-purified

Description: All names and symbols: GRIK3, GLUR7, glutamate receptor, ionotropic, kainate 3, EAA5, GLR7, GluR7a, dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)), excitatory amino acid receptor 5, glutamate receptor 7. Official Symbol: GRIK3. RefSeq number(s): NP_000822.2

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Receptors

Restrictions: For Research Use only

Images

ABIN302159(0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.