ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 10 (ABCB10) antibody

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 10 (ABCB10)

Synonyms: M-ABC2, MTABC2, EST20237, Abc-me, Abcb12, C76534, AU016331, MGC109142, ABCB10, si:dkey-162b3.3

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN302163

Additional Information

Alternative name: ABCB10

Gene ID: 23456, 56199 (mouse), 361439 (rat)

Immunogen: C-KPNGIYRKLMNKQ

Format: Affinity-purified

Description: All names and symbols: ABCB10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, EST20237, M-ABC2, MTABC2, ATP-binding cassette, sub-family B, member 10, ABC-me. Official Symbol: ABCB10. RefSeq number(s): NP_036221.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx. 90kDa and 50kDa in Human Bone Marrow lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 79.1kDa according to NP_036221.2 and given the expected mature size of ~65kDa according to Graf et al. (PMID: 15215243). Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Graf, Haigh, Corson et al.: "Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me)." in: The Journal of biological chemistry, Vol. 279, Issue 41, pp. 42954-63, 2004 (PubMed).