CEBPB antibody (AA 39-78)

Catalog No. ABIN302284

This Mouse Monoclonal antibody specifically detects CEBPB in WB, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CEBPB antibody (AA 39-78) (ABIN302284)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEBPB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 21A1

Product Details anti-CEBPB Antibody

Binding Specificity: AA 39-78

Specificity: This antibody detetects Ccaat/enhancer Binding Protein Beta (CEBPB). Reported to recognize an epitope on NF-IL6 within amino acids 39-78.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: C/EBP beta, TCF5, LAP, NF-IL6, CCAAT/enhancer-binding protein beta, Liver activatorprotein, Nuclear factor NF-IL6, Transcription factor 5

Purification: Protein G Chromatography

Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: CEBPB

Background: NF-IL6 is a member of the CAAT/enhancer-binding protein (C/EBP) family of transcription factors. It has been shown to directly interact with the retinoblastoma (RB) protein.Synonyms: C/EBP beta, CCAAT/enhancer-binding protein beta, LAP, Liver activator protein, NF-IL6, Nuclear factor NF-IL6, TCF5, Transcription factor 5

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation