RPA3 antibody

Catalog No. ABIN302303

The Mouse Monoclonal anti-RPA3 antibody is suitable to detect RPA3 in samples from Human and Mouse. It has been validated for WB, IP and IHC (p).

Quick Overview for RPA3 antibody (ABIN302303)

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 11-01-14

Product Details anti-RPA3 Antibody

Specificity: This antibody detects Replication Protein A3 (14 kDa) (RPA3).

Characteristics: Synonyms: REPA3, RP-A p14, RFA3, RF-A protein 3, Replication protein A, Replication protein A 14 kDasubunit, Replication factor A protein 3

Purification: Protein G column

Immunogen: Full-length recombinant human RPA-14 expressed in E. coli

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for RPA3

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Background: Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.Synonyms: REPA3, RF-A protein 3, RFA3, RP-A p14, Replication factor A protein 3, Replication protein A, Replication protein A 14 kDa subunit

Gene ID: 6119

UniProt: P35244

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA