SGK3 antibody (C-Term)

Catalog No. ABIN302531

The Rabbit Polyclonal anti-SGK3 antibody is suitable to detect SGK3 in samples from Human. It has been validated for IHC (p).

Quick Overview for SGK3 antibody (C-Term) (ABIN302531)

Target: SGK3 (serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SGK3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SGK3 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects Serum/Glucocorticoid Regulated Kinase Family, Member 3 (SGK3/SGKL) at C-terminal domain.

Purification: Immunoaffinity chromatography

Immunogen: SGK3 antibody was raised against synthetic peptide from C-terminal domain of human SGK3.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for SGK3

Target: SGK3 (serum/glucocorticoid Regulated Kinase Family, Member 3 (SGK3))

Alternative Name: SGK3 / SGKL

Background: SGKL/SGK3, also known as CISK for cytokine-independent survival kinase, is a SGK type protein kinase. The protein contains a lipid-binding phox homology (PX) domain, which is required for targeting SGKL/SGK3 protein to the endosomal compartment. SGKL/SGK3 functions downstream of the phosphoinositide 3-kinase (PI3K) cascade and has been implicated in cell survival. The protein has been shown to be phosphorylated and activated by 3-phosphoinositide-dependent protein kinase 1 (PDK1), and to a lesser extent insulin-like growth factor-1, in vitro. Activated SGKL/SGK3 has been shown to phosphorylate glycogen synthase kinase 3 beta (GSK-3beta) in vitro.Synonyms: Serine/threonine-protein kinase Sgk3, Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like

Gene ID: 100533105

NCBI Accession: NP_001028750

UniProt: Q96BR1