CX3CR1 antibody (3rd Extracellular Domain)

Catalog No. ABIN302578

This Rabbit Polyclonal antibody specifically detects CX3CR1 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for CX3CR1 antibody (3rd Extracellular Domain) (ABIN302578)

Target: CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CR1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CX3CR1 Antibody

Binding Specificity: 3rd Extracellular Domain

Specificity: This antinody detects CX3C Chemokine Fractalkine Receptor 1 (CX3CR1) at 3rd extracellular domain.

Purification: Immunoaffinity chromatography

Immunogen: CX3CR1 antibody was raised against synthetic peptide from 3rd extracellular domain of human CX3CR1.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (3.8 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for CX3CR1

Target: CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))

Alternative Name: CX3CR1 / GPR13

Background: CX3CR1 a Chemokine Receptor is an HIV coreceptor, as well as a leukocyte chemotactic/adhesion receptor for fractalkine, also known as neurotactin. CX3CR1 mediates chemotactic responses to the soluble form of fractalkine in neutrophils and T cells, and cytoadhesion to cells expressing membrane-anchored fractalkine, such as monocytes, T cells, and neutrophils. Cells transfected with CX3CR1 exhibited chemotaxis and transient increases in intracellular calcium concentration when exposed to fractalkine.Synonyms: Beta chemokine receptor-like 1, C-X3-C CKR-1, CMK-BRL-1, CMKBRL1, CX3C chemokine receptor 1, Fractalkine receptor, G-protein coupled receptor 13, V28

Gene ID: 1524

NCBI Accession: NP_001164642

UniProt: P49238

Pathways: Cellular Response to Molecule of Bacterial Origin