RAD23B antibody (AA 163-176)

Catalog No. ABIN303137

The Goat Polyclonal anti-RAD23B antibody is suitable to detect RAD23B in samples from Human, Mouse and Rat. It has been validated for WB, IF, IHC (p) and EIA.

Quick Overview for RAD23B antibody (AA 163-176) (ABIN303137)

Target: RAD23B (RAD23 Homolog B (RAD23B))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RAD23B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RAD23B Antibody

Binding Specificity: AA 163-176

Specificity: Recognizes RAD23B (163-176).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 163-176 of Human HR23B protein.

Application Details

Application Notes: ELISA (1/2000-1/10000). Western Blot (1/500-1/2000). Immunofluorescence. Immunohistochemistry on Paraffin Sections (2.5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RAD23B

Target: RAD23B (RAD23 Homolog B (RAD23B))

Alternative Name: RAD23B

Background: HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein

Gene ID: 5887

NCBI Accession: NP_002865

UniProt: P54727

Pathways: DNA Damage Repair