IRF8 antibody (C-Term)

Catalog No. ABIN303274

This Rabbit Polyclonal antibody specifically detects IRF8 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IRF8 antibody (C-Term) (ABIN303274)

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRF8 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognises Interferon Consensus Sequence Binding Protein 1 (IRF8 / ICSBP1).

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic Peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (5 μg/mL). Western Blot (1-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate Buffered Saline PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer. Dilute only prior to immediate use.

Target Details for IRF8

Target: IRF8 (Interferon Regulatory Factor 8 (IRF8))

Alternative Name: IRF8 / ICSBP1

Background: Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemiaSynonyms: IRF-8, Interferon consensus sequence-binding protein, Interferon regulatory factor 8

Gene ID: 3394

NCBI Accession: NP_002154

UniProt: Q02556

Pathways: Cellular Response to Molecule of Bacterial Origin