GLI3 antibody (AA 41-57)

Catalog No. ABIN303380

The Rabbit Polyclonal anti-GLI3 antibody is suitable to detect GLI3 in samples from Human, Rat, Chicken, Dog, Monkey, Quail and Frog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for GLI3 antibody (AA 41-57) (ABIN303380)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Rat, Chicken, Dog, Monkey, Quail, Frog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GLI3 Antibody

Binding Specificity: AA 41-57

Specificity: This antibody recognises GLI-Kruppel Family Member GLI3.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Chicken, Canine (Dog), Frog, Monkey, Quail and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic Peptide -KLH conjugated corresponding to Amino acids 41-57 of Human Gli-3 protein.

Application Details

Application Notes: ELISA (1/6000-1/30000). Western Blot (1/500-1/2000). Immunohistochemistry on Paraffin Sections (1.25 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3

Background: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3

Gene ID: 2737

NCBI Accession: NP_000159

UniProt: P10071

Pathways: Hedgehog Signaling