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MHC Class I HLA-B27 antibody (FITC)

Antigen

MHC Class I HLA-B27

Clonality Monoclonal (HLA-ABC-m3)
Host

Mouse

Reactivity

Human

Conjugate
FITC
Application
Flow Cytometry (FACS)
Catalog no. ABIN304843
Quantity 100 tst
Price 566.67 $   Plus shipping costs $35.00
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Availability Ships within 10 to 15 Business Days

Additional Information

Immunogen Cells, Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Isotype IgG2a
Clone HLA-ABC-m3
Description Gene: MHC Class I HLA-B27
Specificity HLA-ABC-m3 is a cytotoxic antibody Detects HLA-B27 alloantigen. This Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that HLA-B27 has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.

Application Details

Application Notes Flo (1:1)
Purification Ion Exchange Chromatography
Buffer PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA.
Storage +4°C, avoid freezing
Restrictions For Research Use only