V5 Epitope Tag antibody
| Antigen |
|
| Clonality |
Monoclonal (SV5-Pk1) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
Western Blotting (WB) (77), ELISA (54), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (27), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (IHC) (11), Immunocytochemistry (ICC) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Radioimmunoassay (RIA) (5), Dot Blot (Dot) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN304867 |
| Quantity |
1000 µg (1 mg/ml) |
| Price |
606.67 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 10 to 15 Business Days |
|
Alternative name
|
V5 Tag
|
|
Immunogen
|
Virus, Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
|
|
Isotype
|
IgG2a (Matching secondary antibodies)
|
|
Clone
|
SV5-Pk1
|
|
Description
|
Gene: V5 Tag
|
|
Specificity
|
Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr
|
|
Application Notes
|
ELISA (1:1000 - 1:5000), IHC-Fr, IP, RIA, WB (1:1000 - 1:5000)
|
|
Concentration
|
1 mg/ml
|
|
Purification
|
Affinity Chromatography
|
|
Buffer
|
0.09% sodium azide.
|
|
Storage
|
+4°C or -20°C, Avoid repeated freezing and thawing.
|
|
Research Area
|
Tags/Labels
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "V5 Epitope Tag", type "Antibodies"
|
Hosts
|
Mouse (45), Rabbit (44), Goat (9), Chicken (6), Sheep (1)
|
|
Reactivities
|
Tag (16), Fusion Protein (Tag) (14), All Species (7), Virus (2)
|
|
Applications
|
Western Blotting (WB) (77), ELISA (54), Immunofluorescence (IF) (36), Immunoprecipitation (IP) (27), Flow Cytometry (FACS) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (IHC) (11), Immunocytochemistry (ICC) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Radioimmunoassay (RIA) (5), Dot Blot (Dot) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
HRP (11), FITC (9), Biotin (5), Agarose Beads (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
GKPIPNPLLGLDST (10)
|
