VTN / Vitronectin antibody

Antigen: VTN / Vitronectin

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN305725

Additional Information

Swiss-Prot: P04004

Immunogen: Purified protein, Human vitronectin purified from plasma by heparin-affinity chromatography.

Isotype: IgG1, kappa chain  (Matching secondary antibodies)

Description: Gene: VTN / Vitronectin, Standard Gene Symbol: VTN, Family: Matrix Protein, Synonyms: VTN, V75, Vitronectin, VN, VNT

Specificity: Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Cross-reacts with vitronectin from cat, dog, goat, cow and to a lesser extent with horse. Epitope is located in the somatomedin B domain. Binds nearly as well to native vitronectin as to denatured. Is a potent inhibitor of integrin-mediated cell adhesion to vitronectin and a moderate inhibitor of PAI-1 binding. Also binds to vitronectin in ELISA when vitronectin is coated directly onto the microtiter well. Predicted Reactivities: Cow (Bovine),Cat (Feline),Dog (Canine),Goat,Horse (Equine).

Application Details

Application Notes: ELISA (1:30000), IHC-P (2.5 µg/ml), WB (1:50)

Purification: Affinity Chromatography

Buffer: PBS, pH 7.4, 500 mM sodium chloride, 15 mM sodium azide, 40% glycerol.

Storage: Long term: -20°C, Short term: +4°C

Restrictions: For Research Use only

Images

Human Liver (formalin-fixed, paraffin-embedded) stained with VTN antibody ABIN305725 at 2.5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.