SLC9A3R1 antibody

Catalog No. ABIN305897

The Rabbit Polyclonal anti-SLC9A3R1 antibody is suitable to detect SLC9A3R1 in samples from Human and Mouse. It has been validated for WB, IHC, IP and IHC (p).

Quick Overview for SLC9A3R1 antibody (ABIN305897)

Target: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A3R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SLC9A3R1 Antibody

Specificity: Recognizes EBP 50 from human T84 and Calu3 cells as well as recombinant human protein at ~50kD.

Purification: Immunoaffinity purified

Immunogen: Recombinant human His-tagged EBP 50 obtained from bacteria. All His-tag specific antibodies have been removed.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SLC9A3R1

Target: SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))

Alternative Name: SLC9A3R1 / NHERF1 / EBP50

Background: Name/Gene ID: SLC9A3R1
Subfamily: Monovalent cation:proton antiporter-1
Family: Transporter

Synonyms: SLC9A3R1, EBP50, NPHLOP2, NHERF, NHERF-1, NHERF1

Gene ID: 9368

UniProt: O14745

Pathways: Proton Transport, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome