LPAR1 antibody (C-Term)

Catalog No. ABIN306575

The Rabbit Polyclonal anti-LPAR1 antibody is suitable to detect LPAR1 in samples from Human. It has been validated for WB.

Quick Overview for LPAR1 antibody (C-Term) (ABIN306575)

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LPAR1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti Human Lysophosphatidic Acid Receptor 1 CT (EDG-2)

Characteristics: Endothelial cell differentiation gene-2 (EDG-2) receptor is a high affinity receptor for lysophosphatidic acid (LPA). EDG-2, also known as lysophospholipid receptor A1, Vzg-1, mrec1.3 or GPCR26, like all other EDG receptors, couple multiple (3 or more) types of G proteins and transduce decreases in c[AMP]i through Gi and increases in [Ca2+]i by augmenting phospholipase C through Gq/11 and beta/gamma dimers and by induction of PI3 kinase, p125 FAK, phospholipase D by activating rho through G12/13. Human EDG-2 is present in high levels on oligodendrocytes and certain human malignant T cell lines. EDG-2 receptors (with EDG-4) may play a role in protecting cardiomyocytes from apoptosis induced by hypoxia and andrenergic stimulation.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from the C terminal of the EDG2 receptor.

Isotype: IgG

Application Details

Application Notes: For Western blotting, starting dilution of 5-10 μg/mL is recommended. Western blot should be visualized using a high sensitivity secondary/substrate system (i.e. Pierce's SuperSignal West Femto Maximum Sensitivity Substrate). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for LPAR1

Target: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Alternative Name: Lysophosphatidic Acid Receptor 1 CT (EDG-2)

Background: Endothelial Cell Differentiation Gene Receptor 2, Lysophosphatidic Acid Receptor 1

UniProt: Q92633

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration