Bub1 beta antibody

Catalog No. ABIN306788

The Sheep Polyclonal anti-Bub1 beta antibody is suitable to detect Bub1 beta in samples from Mouse. It has been validated for IHC and WB.

Quick Overview for Bub1 beta antibody (ABIN306788)

Target: Bub1 beta

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Purpose: Sheep anti Mouse Bub1 beta-IN

Cross-Reactivity: Mouse

Characteristics: Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

Purification: Ammonium Sulfate Precipitation

Isotype: IgG

Application Details

Application Notes: X1163P antibody detects Bub1b in mitotic NIH/3T3 cells, seperated by immunoprecipitation. Westen blot analysis (1-10 μg/mL) detects a 130 kDa band. Optimal antibody concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: Bub1 beta

Background: Mitotic checkpoint kinase MAD3L

UniProt: Q9Z1S0