S1PR4 antibody

Catalog No. ABIN306801

The Rabbit Polyclonal anti-S1PR4 antibody (ABIN306801) specifically detects S1PR4 in WB. The antibody is reactive with Human samples.

Quick Overview for S1PR4 antibody (ABIN306801)

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-S1PR4 Antibody

Purpose: Rabbit anti Human Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6)

Cross-Reactivity: Human

Characteristics: EDG-6 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-6 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-6 is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG-6 receptor is the sphingosine 1-phosphate receptor-4 (S1P-4).

Purification: Ammonium Sulfate Precipitation

Application Details

Application Notes: Detects EDG6 receptors at concentration of 5-10 μg/mL by Western blot using a human EDG6 receptor transfected cell line. Detects an approximately 45 kDa band in Cos cells transfected with full length human EDG6. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for S1PR4

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Alternative Name: Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6)

Background: Endothelial cell differentiation gene 6 C-terminal, Sphingosine-1-Phosphate Receptor-4 (S1P-4)

UniProt: O95977