Kein 5 &14 antibody

Catalog No. ABIN306822

The Mouse Monoclonal anti-Kein 5 &14 antibody (Clone 16-4) (ABIN306822) specifically detects Kein 5 &14 in IHC (fro) and WB. The antibody is reactive with Human samples.

Quick Overview for Kein 5 &14 antibody (ABIN306822)

Target: Kein 5 &14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Clone: 16-4

Product Details

Purpose: Mouse anti Human Keratin 5 &14

Cross-Reactivity: Human

Characteristics: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Purification: Protein A/G Chromatography

Isotype: IgG2a

Application Details

Application Notes: Detects Keratin 5 and 14 by Western blot. Detects a 58 kDa band, corresponding to Keratin 5, and a 50 kDa band, corresponding to Keratin 14. Antibody can be used for Western blotting and immunohistochemistry on frozen tissue sections (1-5 μg/mL). Does not work on paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details

Target: Kein 5 &14

UniProt: P02533