FA2H antibody

Catalog No. ABIN306946

This Rabbit Polyclonal antibody specifically detects FA2H in WB. It exhibits reactivity toward Human.

Quick Overview for FA2H antibody (ABIN306946)

Target: FA2H (Fatty Acid 2-Hydroxylase (FA2H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FA2H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FA2H Antibody

Purpose: Rabbit anti Human Fatty Acid 2-hydrolase (Fa2H)

Cross-Reactivity: Human

Characteristics: 2-Hydroxysphingolipids are a subset of sphingolipids containing 2-hydroxy fatty acids. The 2-hydroxylation occurs during de novo ceramide synthesis and is catalyzed by fatty acid 2-hydroxylase (also known as fatty acid alpha-hydroxylase). In mammals, 2-hydroxysphingolipids are present abundantly in brain because the major myelin lipids galactosylceramides and sulfatides contain 2-hydroxy fatty acids. Here we report identification and characterization of a human gene that encodes a fatty acid 2-hydroxylase. Data base searches revealed a human homologue of the yeast ceramide 2-hydroxylase gene (FAH1), which we named FA2H. The FA2H gene encodes a 372-amino acid protein with 36 % identity and 46 % similarity to yeast Fah1p. The amino acid sequence indicates that FA2H protein contains an N-terminal cytochrome b5 domain and four potential transmembrane domains. FA2H also contains the iron-binding histidine motif conserved among membrane-bound desaturases/hydroxylases. COS7 cells expressing human FA2H contained 3-20-fold higher levels of 2-hydroxyceramides (C16, C18, C24, and C24:1) and 2-hydroxy fatty acids compared with control cells. Microsomal fractions prepared from transfected COS7 cells showed tetracosanoic acid 2-hydroxylase activities in an NADPH- and NADPH: cytochrome P-450 reductase-dependent manner. FA2H lacking the N-terminal cytochrome b5 domain had little activity, indicating that this domain is a functional component of this enzyme. Northern blot analysis showed that the FA2H gene is highly expressed in brain and colon tissues. These results demonstrate that the human FA2H gene encodes a fatty acid 2-hydroxylase. FA2H is likely involved in the formation of myelin 2-hydroxy galactosylceramides and -sulfatides.

Purification: Ammonium Sulfate Precipitation

Immunogen: Synthetic peptide derived from human fatty acid 2-hydrolase (FA2H) protein.

Application Details

Application Notes: Antibody can be used for Western blotting (5-10 μg/mL) and ELISA. Other applications no tested. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for FA2H

Target: FA2H (Fatty Acid 2-Hydroxylase (FA2H))

Alternative Name: Fatty Acid 2-hydrolase (Fa2H)

Background: Fatty acid 2-hydroxylase, FA2H

UniProt: Q7L5A8