3-Nitrotyrosine antibody

Antigen: 3-Nitrotyrosine

Clonality: Monoclonal (2A12)

Host: Mouse

Reactivity: All Species

Application: Western Blotting (WB)

Catalog no.: ABIN306957

Additional Information

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2A12

Description: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.55 kD 160 kD

Application Details

Application Notes: Antibody can be used for Western blotting (Suggested dilution - 1:1000) and immunohistochemistry.

Concentration: 1 mg/ml

Storage: Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Restrictions: For Research Use only