CYP3A5 antibody (C-Term)

Catalog No. ABIN307112

The Mouse Monoclonal anti-CYP3A5 antibody is suitable to detect CYP3A5 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for CYP3A5 antibody (C-Term) (ABIN307112)

Target: CYP3A5 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 5 (CYP3A5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYP3A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: F18 P3 B6

Product Details anti-CYP3A5 Antibody

Binding Specificity: C-Term

Purpose: Mouse anti Human Cytochrome P450 3A5

Cross-Reactivity: Human

Characteristics: CYP3A genes encode monooxygenases, enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A (cytochrome P450, family 3, subfamily A), the most abundant p450 enzyme in human liver, is responsible for the metabolism of more than 50 % of all clinical drugs. CYP3A members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster consists of four genes: CYP3A43, CYP3A4, CYP3A7 and CYP3A5, and two pseudogenes: CYP3A5P1 and CYP3A5P2. The CYP3A cluster maps to gene locus 7q21.3-q22.1.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human CYP3A5 protein and mouse myeloma Ag8563 cells.

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). When used in conjunction with CYP3A7 (Cat. No. X2048M), can differentiate between the CYP3A family members, 3A5 and 3A7. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CYP3A5

Target: CYP3A5 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 5 (CYP3A5))

Alternative Name: Cytochrome P450 3A5

Background: CYP3A5, CYP450 3A5 (EC 1.14.14.1), Niphedipine oxidase, CYPIIIA5, P450-PCN3, HLp2

UniProt: P20815

Pathways: Steroid Hormone Biosynthesis