CYP26A1 antibody (C-Term)

Catalog No. ABIN307114

This Mouse Monoclonal antibody specifically detects CYP26A1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CYP26A1 antibody (C-Term) (ABIN307114)

Target: CYP26A1 (Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 (CYP26A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYP26A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: F27 P6 A1

Product Details anti-CYP26A1 Antibody

Binding Specificity: C-Term

Purpose: Mouse anti Human Cytochrome p450 26A1

Cross-Reactivity: Human

Characteristics: The cytochrome P450 proteins (CYPs) are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. P450 enzymes are classified into subfamilies based on their sequence similarities. CYP26A1, also referred to as retinoic acid-4-hydroxylase, is a major retinoic acid catabolic enzyme. CYP26A1 plays an important role in protecting tailbud tissues from inappropriate exposure to retinoic acid. CYP26A1 transcription is epigenetically regulated by nuclear retinoic acid receptors 2. Mutations in the gene encoding for CYP26A1 are associated with caudal agenesis and spina bifida, imperforate anus, agenesis of the caudal portions of the digestive and urogenital tracts, and malformed lumbosacral skeletal elements. CYP26A1 is upregulated in adenomatous polyposis coli mouse adenomas, human FAP adenomas, human sporadic colon carcinomas, and in the intestine of adenomatous polyposis coli(mcr) mutant zebrafish embryos.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human CYP450 26A1 protein and mouse myeloma Ag8563 cells.

Isotype: IgG2b

Application Details

Application Notes: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CYP26A1

Target: CYP26A1 (Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 (CYP26A1))

Alternative Name: Cytochrome p450 26A1

Background: CYP450 26 (EC 1.14.-.-), Retinoic acid-metabolizing cytochrome, P450 retinoic acid-inactivating 1, P450RAI, hP450RAI, Retinoic acid 4-hydroxylase

UniProt: O43174

Pathways: Retinoic Acid Receptor Signaling Pathway, Monocarboxylic Acid Catabolic Process