ENO2/NSE antibody

Catalog No. ABIN307127

The Mouse Monoclonal anti-ENO2/NSE antibody is suitable to detect ENO2/NSE in samples from Human, Mouse and Chicken. It has been validated for WB, IHC and ELISA.

Quick Overview for ENO2/NSE antibody (ABIN307127)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human, Mouse, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: NSE-P2

Product Details anti-ENO2/NSE Antibody

Purpose: Mouse anti Human Neuron-Specific Enolase

Cross-Reactivity: Chicken, Human, Mouse

Characteristics: Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, Enolase, Enolase and Enolase. Enolase expression has been detected on most tissues, whereas Enolase is expressed predominantly in muscle tissue and Enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human NSE protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Neuron-Specific Enolase

Background: ENO2, Enolase 2, (gamma, neuronal), 2-Phospho-D-Glycerate Hydrolase

UniProt: P09104