Transglutaminase 2 antibody

Catalog No. ABIN307194

The Rabbit Polyclonal anti-Transglutaminase 2 antibody (ABIN307194) specifically detects Transglutaminase 2 in WB, IHC, IP and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Transglutaminase 2 antibody (ABIN307194)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Transglutaminase 2 Antibody

Specificity: Recognizes human Transglutaminase II at ~77-85kD.

Purification: Protein A purified

Immunogen: Recombinant full length human TGase II (77-85kD). Cellular Localization: Cytoplasm, cell membrane, and extracellular matrix.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB (5 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation (Native verified): 10 μg/mg protein lysate. Use Protein A. Western Blot: 5-10 μg/mL for 2hrs at RT. Positive control: HUVEC cells. Breast carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2 / Transglutaminase 2

Background: Name/Gene ID: TGM2

Synonyms: TGM2, G-ALPHA-h, GNAH, TG(C), Transglutaminase 2, Transglutaminase C, TG2, TGase H, TGase-2, TGase-H, Transglutaminase H, TGase C, TGC, TTG, C polypeptide, Tgase II, Tissue transglutaminase, Transglutaminase-2

Gene ID: 7052

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling