Transglutaminase 2 antibody

Catalog No. ABIN6940725

The Mouse Monoclonal anti-Transglutaminase 2 antibody is suitable to detect Transglutaminase 2 in samples from Human, Mouse, Rat, Monkey and Rabbit. It has been validated for WB and IHC.

Quick Overview for Transglutaminase 2 antibody (ABIN6940725)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TGM2-419

Product Details anti-Transglutaminase 2 Antibody

Purpose: Transglutaminase II (TGM2) Antibody

Specificity: Recognizes a 77-85 kDa protein, identified as cellular or tissue transglutaminase II (TGase II). Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD), a genetic, immunologically mediated small bowel enteropathy that causes malabsorption. TGase II is implicated in programmed cell death, signal transduction, drug-resistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human TGM2 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: HUVEC cells. Endothelial cells in placenta, liver, brain or breast carcinoma. Smooth muscle cells of any origin (e. G. Intestine).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL, 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2

Background:

Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGM2, Tissue type transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase H, tTG, tTGas

Brain, HUVEC cells. Endothelial cells in placenta, Liver, or breast carcinoma. Smooth muscle cells of any origin

Molecular Weight: 77-85kDa

Gene ID: 7052, 517033

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling