Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) antibody

Antigen: Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1)

Synonyms: IRE1, IRE1P, FLJ30999, MGC163277, MGC163279, ERN1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308413

Additional Information

Alternative name: ERN1 / IRE1a

Gene ID: 2081, 78943 (mouse), 498013 (rat)

Immunogen: C-PNAHGKIKAMISD

Format: Affinity-purified

Description: All names and symbols: ERN1, IRE1, endoplasmic reticulum to nucleus signaling 1, FLJ30999, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, endoplasmic reticulum to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease, IRE1a, IRE1-alpha. Official Symbol: ERN1. RefSeq number(s): NP_001424.3

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 110kDa band observed in Human Pancreas lysates (calculated MW of 110kDa according to NP_001424.3). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN308413 (0.03µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Yokouchi, Hiramatsu, Hayakawa et al.: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." in: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, 2008 (PubMed).