ERN1 antibody (C-Term)

Catalog No. ABIN500048

The Rabbit Polyclonal anti-ERN1 antibody is suitable to detect ERN1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and EIA.

Quick Overview for ERN1 antibody (C-Term) (ABIN500048)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ERN1 Antibody

Binding Specificity: C-Term

Specificity: IRE1p antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IRE1p.

Purification: Affinity Chromatography purified via peptide column

Immunogen: 16 amino acid synthetic peptide from near the carboxy terminus of Human IRE1p

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1 / IRE1

Background: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2). These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 (3,4) but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation (5). Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER (6). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, IRE1p, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase/endoribonuclease IRE1

Gene ID: 2081

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response