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Fat Mass and Obesity-Associated (FTO) antibody

Antigen

Fat Mass and Obesity-Associated (FTO)

Synonyms AW743446, mKIAA1752, MGC5149, KIAA1752, RGD1305121, DKFZp459H168
Clonality Polyclonal
Host
Reactivity
Alternatives

Human, Mouse (Murine)

Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN308414
Quantity 100 ug
Price 374.27 $   Plus shipping costs $45.00
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Additional Information

Alternative name FTO
Gene ID 79068, 26383 (mouse), 291905 (rat)
Immunogen QQKPDCRPYWEKDD
Description All names and symbols: Fto, fat mass and obesity associated, AW743446, mKIAA1752, similar to FTO. Official Symbol: Fto. RefSeq number(s): NP_036066.2
Specificity Human, Mouse, Rat, Dog, Pig

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 60kDa band observed in Human Pancreas lysates (calculated MW of 58.0kDa according to NP_036066.2). Recommended concentration: 0.03-0.1µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Gerken, Girard, Tung et al.: "The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase." in: Science (New York, N.Y.), Vol. 318, Issue 5855, pp. 1469-72, 2007 (PubMed).

Alternatives

Alternatives for antigen "Fat Mass and Obesity-Associated (FTO)", type "Antibodies"
Hosts Rabbit (41), Mouse (8), Goat (3), Rat (2)
Reactivities Human (44), Mouse (Murine) (34), Rat (Rattus) (27), Dog (Canine) (19), Cow (Bovine) (18), Horse (Equine) (18), Pig (Porcine) (18), Sheep (Ovine) (18), Monkey (1)
Applications Western Blotting (WB) (38), ELISA (28), Immunofluorescence (IF) (16), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunoprecipitation (IP) (6), Flow Cytometry (FACS) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes N-Term (14), C-Term (3), Center (1), Internal Region (1), N-Term,AA 1-30 (1)