Fat Mass and Obesity-Associated (FTO) antibody

Antigen: Fat Mass and Obesity-Associated (FTO)

Synonyms: AW743446, mKIAA1752, MGC5149, KIAA1752, RGD1305121, DKFZp459H168

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308414

Additional Information

Alternative name: FTO (Mouse)

Gene ID: 79068, 26383 (mouse), 291905 (rat)

Immunogen: QQKPDCRPYWEKDD

Format: Affinity-purified

Description: All names and symbols: Fto, fat mass and obesity associated, AW743446, mKIAA1752, similar to FTO. Official Symbol: Fto. RefSeq number(s): NP_036066.2

Specificity: Human, Mouse, Rat, Dog, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 60kDa band observed in Human Pancreas lysates (calculated MW of 58.0kDa according to NP_036066.2). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN308414 (0.03µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Gerken, Girard, Tung et al.: "The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase." in: Science (New York, N.Y.), Vol. 318, Issue 5855, pp. 1469-72, 2007 (PubMed).