ATP-Binding Cassette, Sub-Family E (OABP), Member 1 (ABCE1) antibody

Antigen: ATP-Binding Cassette, Sub-Family E (OABP), Member 1 (ABCE1)

Synonyms: ABCE1, RLI, Oabp, RNS41, C79080, Rnaseli, Rns4i, abce1, MGC53437, MGC56045, zgc:56045, wu:fb34c09, wu:fe47b01, wu:fi09g07, zgc:111906, MGC69546, MGC139587, DKFZp469K1416, OABP, ABC38, RNS4I, RNASEL1, RNASELI

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN308418

Additional Information

Alternative name: ABCE1 / RNase L

Gene ID: 6059, 24015 (mouse), 361390 (rat)

Immunogen: NHDKCKPKKCRQEC

Format: Affinity-purified

Description: All names and symbols: ABCE1, ATP-binding cassette, sub-family E (OABP), member 1, ABC38, OABP, RLI, RNASEL1, RNASELI, RNS4I, ATP-binding cassette, sub-family E, member 1, RNase L inhibitor, ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) inhibitor. Official Symbol: ABCE1. RefSeq number(s): NP_002931.2, NP_001035809.1

Specificity: Reported variants represent identical protein: NP_002931.2 NP_001035809.1 Human, Mouse, Rat, Dog, Cow

Synonyms: ABCE1, RLI, Oabp, RNS41, C79080, Rnaseli, Rns4i, abce1, MGC53437, MGC56045, zgc:56045, wu:fb34c09, wu:fe47b01, wu:fi09g07, zgc:111906, MGC69546, MGC139587, DKFZp469K1416, OABP, ABC38, RNS4I, RNASEL1, RNASELI

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx. 80kDa band in Human Spleen lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.3kDa according to NP_002931.2. The ~80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Shichijo, Ishihara, Azuma et al.: "ABCE1, a member of ATP-binding cassette transporter gene, encodes peptides capable of inducing HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes in colon cancer patients." in: Oncology reports, Vol. 13, Issue 5, pp. 907-13, 2005 (PubMed).