SAR1 Homolog A (S. Cerevisiae) (SAR1A) antibody

Antigen: SAR1 Homolog A (S. Cerevisiae) (SAR1A)

Synonyms: SAR1, Sara, SARA1, masra2, SAR1A, MGC127517, LOC595115, T14P4.21, T14P4_21, DKFZp470L1214, sar1a, LOC100283241

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308431

Additional Information

Alternative name: SAR1

Gene ID: 56681, 20224 (mouse), 361842 (rat)

Immunogen: C-HSRLVESKVELNA

Description: All names and symbols: SAR1A, SAR1 gene homolog A (S. cerevisiae), SAR1, SARA1, Sara, masra2, OTTHUMP00000060603, SAR1a gene homolog 1. Official Symbol: SAR1A. RefSeq number(s): NP_064535.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 23 kDa band observed in lysates of NIH3T3 (calculated MW of 22.4 kDa according to Human NP_064535.1 and to Mouse NP_033146.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN308431 (0.1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Bi, Mancias, Goldberg: "Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31." in: Developmental cell, Vol. 13, Issue 5, pp. 635-45, 2007 (PubMed).