Clusterin / ApoJ (mouse) antibody

Antigen: Clusterin / ApoJ (mouse)

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308436

Additional Information

Gene ID: 12759 (mouse), 24854 (rat)

Immunogen: C-EKALQEYRRKSRAE

Format: Affinity-purified

Description: All names and symbols: ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message. Official Symbol: Clu. RefSeq number(s): NP_038520.1

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 37kDa band observed in Mouse Brain and Testis lysates and in Rat Brain and Ovary lysates (calculated MW of 51.6kDa according to Mouse NP_038520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18,104(12):1407-12., PMID: 11560857). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN308436 (0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Charnay, Imhof, Vallet et al.: "Clusterin expression during fetal and postnatal CNS development in mouse." in: Neuroscience, Vol. 155, Issue 3, pp. 714-24, 2008 (PubMed).