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CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae) (SEPT7) (internal) antibody

Antigen

CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae) (SEPT7)

Synonyms CDC3, CDC10, SEPT7A, Nbla02942
Epitope

internal

Clonality Polyclonal
Host

Goat

Reactivity

Human

Application
ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN308440
Quantity 100 ug
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name Septin-7 / SEPT7 (Internal)
Gene ID 989, 235072 (mouse), 64551 (rat)
Immunogen C-DNNKNKGQLTKSP
Format Affinity-purified
Description All names and symbols: SEPT7, septin 7, CDC10, CDC3, Nbla02942, SEPT7A, CDC10 (cell division cycle 10, S. cerevisiae, homolog), CDC10 cell division cycle 10 homolog, cell division cycle 10. Official Symbol: 01.09.07. RefSeq number(s): NP_001779.3, NP_001011553.2
Specificity This antibody is expected to recognize both reported isoforms (NP_001779.3, NP_001011553.2). Human, Mouse, Rat, Cow, Horse, Dog

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.7kDa according to NP_001779.3 and of 55.6kDa according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Kremer, Adang, Macara: "Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7." in: Cell, Vol. 130, Issue 5, pp. 837-50, 2007 (PubMed).