Dolichyl-Phosphate Mannosyltransferase Polypeptide 1, Catalytic Subunit (DPM1) antibody

Antigen: Dolichyl-Phosphate Mannosyltransferase Polypeptide 1, Catalytic Subunit (DPM1)

Synonyms: dpm1, DPM1, zgc:101018, MGC140708, MPDS, CDGIE, MGC114892

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308441

Additional Information

Alternative name: DPM1

Gene ID: 8813

Immunogen: RELEVRSPRQNKYS-C

Format: Affinity-purified

Description: All names and symbols: DPM1, dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, CDGIE, MPDS, dolichol monophosphate mannose synthase, dolichyl-phosphate mannosyltransferase polypeptide 1. Official Symbol: DPM1. RefSeq number(s): NP_003850.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.6kDa according to NP_003850.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Organelles

Restrictions: For Research Use only

Images

ABIN308441 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ashida, Maeda, Kinoshita: "DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3." in: The Journal of biological chemistry, Vol. 281, Issue 2, pp. 896-904, 2006 (PubMed).