Component of Oligomeric Golgi Complex 7 (COG7) antibody

Antigen: Component of Oligomeric Golgi Complex 7 (COG7)

Synonyms: CDG2E, Gm167, 5630400E24Rik, Cog7, MGC124861, MGC80865, COG7, MGC107838, MGC152912, zgc:152912, zgc:153470, DKFZp469B0824

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN308445

Additional Information

Alternative name: COG7

Gene ID: 91949

Immunogen: C-KTRPEDYRQVSK

Format: Affinity-purified

Description: All names and symbols: COG7, component of oligomeric golgi complex 7, CDG2E. Official Symbol: COG7. RefSeq number(s): NP_705831.1

Specificity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 85kDa band observed in lysates cell lines HeLa, Jurkat and MOLT4 (calculated MW of 86.3kDa according to NP_705831.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN308445 (0.03µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Oka, Vasile, Penman et al.: "Genetic analysis of the subunit organization and function of the conserved oligomeric golgi (COG) complex: studies of COG5- and COG7-deficient mammalian cells." in: The Journal of biological chemistry, Vol. 280, Issue 38, pp. 32736-45, 2005 (PubMed).