Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G) antibody

Antigen: Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G)

Synonyms: PHA1, ENaCg, SCNEG, ENaCgamma, ENaC, SCNN1G, scnn1g, MGC81424

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN308448

Additional Information

Alternative name: SCNN1G

Gene ID: 6340

Immunogen: C-SNQLTDTQMLDE

Format: Affinity-purified

Description: All names and symbols: SCNN1G, sodium channel, nonvoltage-gated 1, gamma, ENaCg, ENaCgamma, PHA1, SCNEG, ENaC gamma subunit, amiloride-sensitive epithelial sodium channel gamma subunit. Official Symbol: SCNN1G. RefSeq number(s): NP_001030.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 140kDa band in Human Breast cancer and Colon lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.3kDa according to NP_001030.2. The 140kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Boyd, Naray-Fejes-Toth: "Steroid-mediated regulation of the epithelial sodium channel subunits in mammary epithelial cells." in: Endocrinology, Vol. 148, Issue 8, pp. 3958-67, 2007 (PubMed).