SCNN1G antibody (AA 80-401)

Catalog No. ABIN7427612

This Mouse Monoclonal antibody specifically detects SCNN1G in WB and IHC. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for SCNN1G antibody (AA 80-401) (ABIN7427612)

Target: SCNN1G (Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCNN1G antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C1

Product Details anti-SCNN1G Antibody

Binding Specificity: AA 80-401

Purpose: Monoclonal Antibody to Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g)

Specificity: The antibody is a mouse monoclonal antibody raised against SCNN1g. It has been selected for its ability to recognize SCNN1g in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Amiloride Sensitive Sodium Channel Subunit Gamma (SCNN1g) corresdonding to Thr80~Thr401 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-SCNN1G antibody (ABIN7427612)

Sudarikova, Bychkov, Kulbatskii, Chubinskiy-Nadezhdin, Shlepova, Shulepko, Koshelev, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer." in: Frontiers in oncology, Vol. 12, pp. 904742, (2022) (PubMed).

Bychkov, Kirichenko, Shulepko, Mikhaylova, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Growth, Migration, and Invasion of Metastatic Melanoma Cells by Targeting the Channels Containing an ASIC1a Subunit Whose Up-Regulation Correlates with Poor Survival Prognosis." in: Biomedicines, Vol. 9, Issue 10, (2021) (PubMed).

Target Details for SCNN1G

Target: SCNN1G (Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G))

Alternative Name: SCNN1g

Background: ENaCg, ENaCgamma, PHA1, SCNEG, Sodium Channel,Nonvoltage-Gated 1,Alpha, Epithelial Na(+) channel subunit gamma, Nonvoltage-gated sodium channel 1 subunit gamma

UniProt: P51170