ZIC2 (ZIC2) antibody

Antigen: ZIC2 (ZIC2)

Synonyms: HPE5

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN309795

Additional Information

Alternative name: ZIC2

Gene ID: ZIC2

Immunogen: The immunogen for anti-ZIC2 antibody: synthetic peptide directed towards the C terminal of human ZIC2

Sequence: EPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGA GGGSGGGSGS

Cross-Reactivity: Human

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-ZIC2 antibody concentration is 1 mg/ml.

Description: ZIC2 is a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects.This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13.

Characteristics: Antigen Size: 532 amino acids

Molecular Weight: 55kDa

Application Details

Application Notes: WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate

anti-ZIC2 (ZIC2) antibody (Image 2)

Publications

Product: Brown, Paraso, Arkell et al.: "In vitro analysis of partial loss-of-function ZIC2 mutations in holoprosencephaly: alanine tract expansion modulates DNA binding and transactivation." in: Human molecular genetics, Vol. 14, Issue 3, pp. 411-20, 2005 (PubMed).

McCampbell, Truong, Broom et al.: "Mutant SPTLC1 dominantly inhibits serine palmitoyltransferase activity in vivo and confers an age-dependent neuropathy." in: Human molecular genetics, Vol. 14, Issue 22, pp. 3507-21, 2005 (PubMed).