DEAF1 antibody (N-Term)

Catalog No. ABIN309945

The Rabbit Polyclonal anti-DEAF1 antibody (ABIN309945) specifically detects DEAF1 in WB. The antibody is reactive with Human, Mouse, Rat, Guinea Pig, Dog, Cow and Saccharomyces cerevisiae samples.

Quick Overview for DEAF1 antibody (N-Term) (ABIN309945)

Target: DEAF1 (Deformed Epidermal Autoregulatory Factor 1 (Drosophila) (DEAF1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DEAF1 Antibody

Binding Specificity: N-Term

Purpose: DEAF1 Antibody - N-terminal region

Sequence: EEPVLSRDED SEEDADSEAE RETPRVTAVA VMAAEPGHMD MGAEALPGPD

Predicted Reactivity: Cow: 83%, Dog: 93%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Yeast: 100%

Characteristics: This is a rabbit polyclonal antibody against DEAF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DEAF1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098708

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DEAF1

Target: DEAF1 (Deformed Epidermal Autoregulatory Factor 1 (Drosophila) (DEAF1))

Alternative Name: DEAF1

Background:

Background Information: DEAF1 down-regulates transcription of those genes by binding to sequence with multiple copies of TTC[CG]G present in their own promoter and that of the HNRPA2B1 gene. DEAF1 binds to the retinoic acid response element (RARE) AGGGTTCACCGAAAGTTCA. DEAF1 activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase.

Gene Name: Deformed epidermal autoregulatory factor 1 (Drosophila)

Alternative Symbols: SPN, NUDR, VSVS, MRD24, ZMYND5, NEDHELS

Protein Name: Deformed epidermal autoregulatory factor 1 homolog

Molecular Weight: 59kDa

Gene ID: 10522

NCBI Accession: NP_066288

UniProt: O75398

Pathways: Tube Formation