EIF4E3 antibody (Middle Region)

Catalog No. ABIN310121

There is 1 publication for this product available. The Rabbit Polyclonal anti-EIF4E3 antibody is suitable to detect EIF4E3 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for EIF4E3 antibody (Middle Region) (ABIN310121)

Target: EIF4E3 (Eukaryotic Translation Initiation Factor 4E Family Member 3 (EIF4E3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4E3 Antibody

Binding Specificity: Middle Region

Purpose: EIF4E3 Antibody - middle region

Sequence: VQVWNVNASL VGEATVLEKI YELLPHITFK AVFYKPHEEH HAFEGGRGKH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4E3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4E3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099993

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF4E3 antibody (ABIN310121)

Yi, Papadopoulos, Hagner, Wagner: "Hypoxia-inducible factor-1? (HIF-1?) promotes cap-dependent translation of selective mRNAs through up-regulating initiation factor eIF4E1 in breast cancer cells under hypoxia conditions." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 18732-42, (2013) (PubMed).

Target Details for EIF4E3

Target: EIF4E3 (Eukaryotic Translation Initiation Factor 4E Family Member 3 (EIF4E3))

Alternative Name: EIF4E3

Background:

Background Information: EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome.

Gene Name: Eukaryotic translation initiation factor 4E family member 3

Alternative Symbols: eIF-4E3, eIF4E-3

Protein Name: Eukaryotic translation initiation factor 4E type 3

Molecular Weight: 24kDa

Gene ID: 317649

NCBI Accession: NP_001128123

UniProt: Q8N5X7