MAT1A antibody (C-Term)

Catalog No. ABIN310139

There is 1 publication for this product available. The Rabbit Polyclonal anti-MAT1A antibody is suitable to detect MAT1A in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for MAT1A antibody (C-Term) (ABIN310139)

Target: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAT1A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAT1A Antibody

Binding Specificity: C-Term

Purpose: MAT1A Antibody - C-terminal region

Sequence: VAKSLVKAGL CRRVLVQVSY AIGVAEPLSI SIFTYGTSQK TERELLDVVH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against MAT1A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MAT1A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP41399-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAT1A antibody (ABIN310139)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Target Details for MAT1A

Target: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Alternative Name: MAT1A

Background:

Background Information: MAT1A catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. MAT1A is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in its gene are associated with methionine adenosyltransferase deficiency.This gne encodes methionine adenosyltransferase I (alpha isoform), which catalyzes the formation of S-adenosylmethionine from methionine and ATP. Methionine adenosyltransferase deficiency is caused by recessive and dominant mutations, the latter identified in autosomal dominant persistant hypermethioninemia.

Gene Name: Methionine adenosyltransferase I, alpha

Alternative Symbols: MAT, SAMS, MATA1, SAMS1

Protein Name: S-adenosylmethionine synthase isoform type-1

Molecular Weight: 44kDa

Gene ID: 4143

NCBI Accession: NP_000420

UniProt: Q00266

Pathways: Mitotic G1-G1/S Phases, M Phase, Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process