Aminolevulinate Dehydratase (ALAD) antibody
| Antigen |
|
| Synonyms |
PBGS, ALADH, MGC5057, Lv, ALADR, aminolevulinatedelta-dehydratase, ALAD, ncf, zgc:110219, CH73-203I15.1 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
 Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
|
|
Conjugate
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
|
|
Application
|
|
| Catalog no. |
ABIN310192 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 2 to 3 Business Days |
|
Alternative name
|
ALAD
|
|
Gene ID
|
ALAD
|
|
UniProt
|
Q6ZMU0
|
|
Immunogen
|
The immunogen for anti-ALAD antibody: synthetic peptide directed towards the middle region of human ALAD
|
|
Sequence
|
SVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGAR GLALRAVDRD
|
|
Cross-Reactivity
|
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
|
|
Format
|
Lyophilized
|
|
Reconstitution
|
Add 50 µl of distilled water. Final anti-ALAD antibody concentration is 1 mg/ml.
|
|
Description
|
The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.
|
|
Characteristics
|
Antigen Size: 359 amino acids
|
|
Molecular Weight
|
39kDa
|
|
Application Notes
|
WB Suggested Anti-ALAD Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate.
|
|
Purification
|
Affinity Purified
|
|
Buffer
|
PBS
|
|
Storage (Long Term)
|
For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Storage (Short Term)
|
Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
|
|
Restrictions
|
For Research Use only
|
|
Product
|
Tang, Breinig, Stith et al.: "Single amino acid mutations alter the distribution of human porphobilinogen synthase quaternary structure isoforms (morpheeins)." in: The Journal of biological chemistry, Vol. 281, Issue 10, pp. 6682-90, 2006 (PubMed).
|
Alternatives for antigen "Aminolevulinate Dehydratase (ALAD)", type "Antibodies"
|
Hosts
|
Rabbit (33)
|
|
Reactivities
|
Human (29), Mouse (Murine) (21), Cow (Bovine) (19), Rat (Rattus) (19), Dog (Canine) (1), Xenopus laevis (1), Zebrafish (1)
|
|
Applications
|
Western Blotting (WB) (18), Immunofluorescence (IF) (14), ELISA (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
|
|
Epitopes
|
N-Term (6), C-Term (2), C-Term,AA 251-280 (1), Internal Region (1)
|
